Olivier Lardinois, PhD
Specialty Areas: Mass spectrometry; proteomics; inflammation; reactive oxygen/nitrogen species; glycans; immunoglobulins; peroxidases; spin-trapping.
Chronology: BS: University of Louvain (Belgium), 1985; Undergraduate researcher: University of Louvain, 1985-1991; PhD: University of Louvain, 1997; Post-doctoral fellow: University of California San Francisco, 1997-2000; Postgraduate researcher: University of California San Francisco, 2001-2003; Research fellow: National Institutes of Health, NIEHS, 2003-2012; Associate Professor: University of North Carolina, 2012-Present.
Dr. Lardinois’ major research interest lies in the field of mass spectrometry and its application to the analysis of antigens/antibodies involved in a variety of renal auto-immune diseases, with a special emphasis on ANCA (AntiNeutrophilic Cytoplasmic Autoantibodies) and autoimmune vasculitides (inflammations of blood vessels). Antibodies are major components of the immune systems and ANCAs are a group of autoantibodies directed against constituents of neutrophil granulocytes (the most common type of white blood cells) and monocytes (another type of white blood cell). A body of evidence point to a crucial role for ANCA in the disease process for several types of small-vessel vasculitides; Wegener's granulomatosis, Churg-Strauss syndrome, and Microscopic polyangiitis. Intriguingly, however, ANCA levels do not correlate or correlate only moderately with disease activity. ANCAs are normally of the IgG type. IgGs are glycoproteins (molecules that consist of a carbohydrate – a “sugar”- plus a protein). Typically, more than 30 IgG glycovariants (molecular forms varying in their carbohydrate composition and structure) can be detected in human serum and the proportion of individual IgG glycovariants in patients with active ANCA-associated vasculitides differ from healthy individual. Dr. Lardinois is currently investigating whether changes in the abundance of certain glycovariants of ANCA could affect their pathogenicity and be correlated with disease activity. Another focus is the development of LC-MS/MS based methods to identify and characterize new antigens/antibodies involved in renal auto-immune diseases.